A multi-dimensional detection system is adopted to confirm the characteristics of exosomes: Transmission electron microscopy (TEM) is utilized to observe their characteristic structures, nanoparticle analyzers or nano-flow cytometers are employed to detect particle sizedistribution, and Western blot is conducted to verify marker proteins such as CD9/CD63. Meanwhile, sequencing technology is applied to analyze nucleic acid composition, and mass spectrometry is used to identify protein components, ensuring that exosome samples conform to international research standards and providing a reliable data foundationfor subsequent experiments.