A multi-dimensional detection system is employed to confirm the characteristics of exosomes: transmission electron microscopy (TEM) is used to observe their characteristicstructures, nanoparticle analyzers or nano-flow cytometers are utilized to detect particle sizedistribution, and Western blot is performed to verify marker proteins such as CD9/CD63. Simultaneously, sequencing technology is applied to analyze nucleic acid composition, andmass spectrometry is used to identify protein components, ensuring that exosome samples meet international research standards and providing a solid data foundation for subsequent experiments.